Coding

Part:BBa_K1039030:Design

Designed by: Urooj Kishor   Group: iGEM13_Waterloo   (2013-09-12)


PhiC31 integrase with Promoter and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1726
    Illegal SapI.rc site found at 1417
    Illegal SapI.rc site found at 1475
    Illegal SapI.rc site found at 1580


Design Notes

Added base after the stop codon to prevent creation of an SpeI site due to addition of suffix


Source

The DNA was synthesized by New England BioLabs

References